Uji Toksisitas Jamur Metarhizium anisopliae terhadap Hama Ulat Krop Kubis Crocidolomia binotalis Zell.

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Siska Agustina Pertiwi

Universitas Jember

Nanang Tri Haryadi

Universitas Jember

Abstract

Cabbage head caterpillar Crocidolomia binotalis Zell. caused up to 79.81% damage to cabbage plants. Metarhizium anisopliae is an entomopathogenic fungus that is often used for pest control in the field because it is toxic to various phases of insect life. This study aimed to determine the toxicity ability of the fungus Metarhizium anisopliae in controlling the population of the cabbage head caterpillar Crocidolomia binotalis Zell. in the laboratory. The research was conducted at the Laboratory of Agrotechnology, Faculty of Agriculture, University of Jember. The study used a Completely Randomized Design with 5 treatments and 4 replications in order to obtain 20 experimental units. The treatments used in this study were as follows: A = Control (aquadest); B = density 104 conidia/ml; C = density 105 conidia/ml; D = 106 conidia/ml; and E = 107 conidia/ml. The results showed that in the laboratory test the density of the fungus Metarhizium anisopliae 107 conidia/ml caused the highest mortality of 92.50%, while the lowest mortality percentage was 22.50% in the treatment of 104 conidia/ml on 7 days of observation. The results of the LT50 calculation showed that the most effective density was 106 conidia/ml with an LT50 value on the 4th day. The most effective density of Metarhizium anisopliae, 106 conidia/ml, was recommended for use in population control of the cabbage head caterpillar Crocidolomia binotalis Zell. because it is capable of causing the death of 50% of the population of Crocidolomia binotalis Zell. with the fastest time of about 4 days.

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Ahmad, R. Z. (2004). Cendawan Metarhizium anisopliae sebagai pengendali hayati ektoparasit caplak dan tungau pada ternak. Balai Penelitian Veteriner, Bogor.

Athifa, S., Anwar, S., dan Kristanto, B. A. (2018). Pengaruh keragaman jamur Metarhizium anisopliae terhadap mortalitas larva hama Oryctes rhinoceros dan Lepidiota stigma. Agro Complex, 2(2), 120-127.

[BSN] Badan Standarisasi Nasional. (2014). SNI 8027. 2: 2014. Agens Pengendali Hayati (APH) – Bagian 2: Metarhizium anisopliae. Badan Standarisasi Nasional: Jakarta

Butt, T.M., C. Jackson, & N. Magan. (2001). Fungi as biocontrol agents. CABI Publishing.

Gabriel, B., & Riyatno, P. (1989). Metarhizium anisopliae (Meetsch) Sor. taksonomi, patologi, produksi dan aplikasinya. Proyek Pengembangan Perlindungan Tanaman Perkebunan, Departemen Pertanian, Jakarta.

Gustiana, D., Hidayat, C. & Setiati, Y. (2019). Pengendalian Plutella xylostella dengan Metarhizium anisopliae dalam mengurangi intensitas kerusakan dan mempertahankan hasil kubis. Agroscript, 1(1), 21-28.

Halawa, B., Azwana, & Panggabean, E. L. (2019). Sensitivity of larva Spodoptera litura against the density of spores of fungi Metarhizium anisopliae on the onion plant red (Allium cepa) in the laboratory. Budapest International Research in Exact Sciences (BirEx) Journal, 1(1), 35-41.

Hastuti, D., Rusbana, T. B., & Hidayatullah, D. N. (2017). Pengaruh lama penyimpanan jamur Metarhizium anisopliae terhadap mortalitas ulat grayak Spodoptera litura F. di laboratorium. Agroekotek, 9(1), 17-27.

Hasyim, A., Setiawati, W., Hudayya, A. & Luthfy. (2016). Sinergisme jamur entomopatogen Metarhizium anisopliae dengan insektisida kimia untuk meningkatkan mortalitas ulat bawang Spodoptera exigua. Hort, 26(2), 257-266.

Huang, P., Yao, J., Lin, Y. & Yu, D. (2021). Pathogenic characteristics and infection-related genes of Metarhizium anisopliae FM-03 infecting Planococcus lilacinus. Entomologia Experimentalis et Applicata, 169, 437-448.

Khreb W. A. A. (2004). Virulence bio-assay efficiency of Beauferia bassiana and Metarhizium anisopliae for the biological control of Spodoptera exigua Hubner (Lepidoptera: Noctuidae) eggs and the 1st instar larvae. J. Basic & Appl. Sci., 8(3), 313-323.

Kristanto, S. P., Sutjipto, & Soekarno. (2013). Pengendalian hama pada tanaman kubis dengan sistem tanam tumpangsari. Berkala Ilmiah Pertanian, 1(1), 7-9.

Mondal, S., Baksi, S., Koris, A., & Vatai, G. (2016). Journey of enzymes in entomopathogenic fungi. Pacific Science Review A: Natural Science and Engineering, 18, 85-99.

Nisfuriah, L. & Nunilahwati, H. (2020). Uji pertumbuhan koloni jamur entomopatogen dari pertanaman kacang panjang (Vigna Sinensis L.) pada serangga umpan. Journal of Global Sustainable Agriculture (JGSA), 1(1), Edisi 1, 9-13.

Nuraida & Hasyim, A. (2009). Isolasi, identifikasi, dan karakterisasi jamur entomopatogen dari rizosfir pertanaman kubis. J. Hort, 19(4), 419-432.

Nuraida, Hutagaol, D., & Hariani, F. (2022). Monograf Persistensi dan Transmisi Isolat Jamur Entomopatogen Metarhizium anisopliae: Pengendalian Hama Kubis Crocidolomia pavonana Fabricus. Medan: Guepedia.

Olombrada, M., Medina, P., Budia, F., Gavilanes, J. G.,Martinez del Pozo, A. & Garcia Ortega, L. (2017). Characterization of a new toxin from the entomopathogenic fungus Metarhizium anisopliae: the ribotoxin anisoplin. Biol. Chem., 398(1), 135-142.

Permadi, M. A., Lubis, R. A. & Siregar, I. K. (2019). Studi keragaman cendawan entomopatogen dari berbagai rizosfer tanaman hortikultura di kota Padangsidimpuan. Penelitian dan Pembelajaran MIPA, 4(1), 1-9.

Prayogo, Y. & Tengkano, W. (2002b). Pengaruh umur larva Spodoptera litura terhadap efektivitas Metarhizium anisopliae isolat Kendalpayak. Majalah Ilmiah Biologi Biosfera, (19)3, 70-76.

Prayogo, Y., Tengkano, W. & Marwanto. (2005). Prospek cendawan entomopatogen Metarhizium anisopliae untuk mengendalikan ulat grayak Spodoptera litura pada tanaman kedelai. Litbang Pertanian, 24(1), 19-26.

Rahmawati, R. (2012). Cepat & Tepat Berantas Hama & Penyakit Tanaman. Yogyakarta: Pustaka Baru Press.

Rusdy, A. (2009). Efektivitas ekstrak nimba dalam pengendalian ulat grayak (Spodoptera litura F.) pada tanaman selada. Floratek, 4, 41-54.

San Aw, K. M., & Hue, S. M. (2017). Mode of infection of Metarhizium spp. fungus and their potential as biological control agents. J. Fungi, 3(30), 1-20.

Sanjaya, Y., Ocampo, V.R. & Caoili, B. L. (2013). Infection process of entomopathogenic fungi Metarhizium anisopliae in the Tetrancyhus kanzawai (Kishida) (Tetranychidae: Acarina). Agrivita, 35(1), 64-73.

Sastrosiswojo, S., Uhan, T. S. & Sutarya, R. (2005). Penerapan teknologi pht pada tanaman kubis. Bandung: Balai Penelitian Tanaman Sayuran.

Sayuthi, M., Hasnah, Rusdy, A. & Alwi. (2020). The effectiveness of the fungus Metarhizium anisopliae as a biocontrol agent against the Nezara viridula pest in the province of Aceh. IOSR Journal of Agriculture and Veterinary Science, 12(2), 27-32.

Tiago, P. V., Oliveira, N. T. & Lima, E. A. de L. A. (2014). Biological insect control using Metarhizium anisopliae: morphological, molecular, and ecological aspects. Ciência Rural, Santa Maria, 44(4), p. 645-651.

Tobing, S. S. L., Marheni, & Hasanuddin. (2015). Uji efektivitas Metarhizium anisopliae Metch. dan Beauveria bassiana Bals. terhadap ulat grayak (Spodoptera litura F.) pada tanaman kedelai (Glicyne max L.) di rumah kassa. Agroteknologi, 4(1), 1659-1665.

Yuningsih & Widyaningrum, T. (2014). Uji patogenitas konidia jamur Metarhizium anisopliae terhadap mortalitas larva Oryctes rhinoceros sebagai bahan ajar biologi SMA kelas X. JUPEMASI-PBIO, 1(1), 53-59.

Yunizar, N., Rahmawati & Kustiati. (2018). Patogenesitas isolat jamur Metarhizium anisopliae terhadap lalat rumah Musca domestica L. (Diptera: Muscidae). Protobiont, 7(3), 77-82.

Siska Agustina Pertiwi, Universitas Jember

Program Studi Agroteknologi, Fakultas Pertanian

Nanang Tri Haryadi, Universitas Jember

Program Studi Agroteknologi, Fakultas Pertanian
Title Uji Toksisitas Jamur Metarhizium anisopliae terhadap Hama Ulat Krop Kubis Crocidolomia binotalis Zell.
Issue: Vol. 23 No. 2 (2022): JURNAL AGRI-TEK
Section Articles
Published: Oct 24, 2022
DOI: https://doi.org/10.33319/agtek.v23i2.116
Keywords: Crocidolomia binotalis Zell, cabbage, Metarhizium anisopliae, mortality
Author
  • Siska Agustina Pertiwi
    Universitas Jember
    Program Studi Agroteknologi, Fakultas Pertanian
  • Nanang Tri Haryadi
    Universitas Jember
    Program Studi Agroteknologi, Fakultas Pertanian
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How to Cite
Pertiwi, S. A., & Nanang Tri Haryadi. (2022). Uji Toksisitas Jamur Metarhizium anisopliae terhadap Hama Ulat Krop Kubis Crocidolomia binotalis Zell. JURNAL AGRI-TEK : Jurnal Penelitian Ilmu-Ilmu Eksakta, 23(2), 15–20. https://doi.org/10.33319/agtek.v23i2.116
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