Isolasi Jamur Selulolitik Trichoderma pada Beberapa Limbah Organik

Keywords:

Agus Purwanto

Universitas Katolik Widya Mandala Surabaya Kampus Kota Madiun

Abstract

Cellulolytic fungi have an important role for the largest material flow in the biosphere. Trichoderma fungi are ubiquitous and have the ability to degrade cellulose. The study was conducted to isolate dan screen Trichoderma cellulolytic fungi from soil heaps of some organic waste. Soil samples were taken from soil heaps of some organic waste to isolate Trichoderma cellulolytic fungi. The sampling method is carried out randomly from top soil layers of organic waste litter (bagasse, sugar cane leaf, rice straw, and garbage). Isolation and screening of Trichoderma fungi are determined based on observations of macroscopic and microscopic characteristics referring to the key of fungal determination. Cellulolytic ability of Trichoderma isolates was carried out by growing on Carboxy Methyl Cellulose (CMC) media as a single carbon source by measuring the diameter of the colony and the diameter of the clear zone formed. There were 7 Trichoderma fungal isolates  that showed cellulolytic activity. Based on clear zone formation, Trichoderma harzianum showed highest cellulolytic than Trichoderma viride.

Downloads

Download data is not yet available.

Barnett, HL and BB, Hunter. 1972, Illustrated genera of imperfect fungi , 3rd edition, Burgess Publishing Co.

Carreiro, M. M. And Koske, R. E. 1992. Effect of Temperature on Decomposition and Development of Microfungal Communities in Leaf Litter Microcosms. Canadian Journal of Botany 70, 2177-2183.

Collins, R.P. and A.F. Hallim. 1972. Characterization of major Aroma Constituents of the Fungus Trichoderma viride. (Pers). J. Agric. Food Chem. 20 (2): p. 437-438.

Domsch, KM., W. Gams and TH. Anderson. 1980. Compendium of Soil Fungi, Vol. 1. Academic Press Inc., New York: 1-391.

Gams, W. and Bissett, J. 1998. Morphology and Identification of Trichoderma. In Trichoderma and Gliocladium, pp. 3-31. Edited by C. P. Kubicek & G. E. Harman. London; Bristol, PA: Taylor & Francis.

Gautam, C., and Gupta, S. 2014. Antagonistic Effect of Trichoderma viride and Trichoderma harzianum against plant pathogenic fungi and its growth on different agro-waste substrates. National Conference on Synergetic Trends in Engineering and Technology (STET-2014), 23-24

Garbeva, P., van Veen, J. A. and van Elsas, J.D. 2004. Microbial Diversity in Soil: Selection of Microbial Populations by Plant and Soil Type and Implications for Disease Suppressiveness. Annual Review of Phytopathology 42:243-270.

Hagn, A., Pritsch, K., Schloter, M. and Munch, J.C. 2003. Fungal Diversity in Agricultural Soil under Different Farming Management Systems, with Special Reference to Biocontrol Strains of Trichoderma spp. Biology & Fertility of Soils 38: 236-244.

Hoorman, JJ and R. Islam, R. 2010. Understanding Soil Microbes and Nutrient Recycling. Agriculture and Natural Resources, Ohio State University.

Junaid, M. 2006. Kemampuan Cendawan Trichoderma sp. Menghasilkan Enzim Kitinase, B-1,3 Glukanase, Kutinase serta Daya Tumbuh In Vitro di Permukaan Bunga dan Buah Kakao. Tesis. SSP. PPS Unhas, Makasar.

Irawadi, T. 1990. Selulase. PAU Biotek. Institut Pertanian Bogor. Bogor.

Kader AJ dan O. Omar. 1998. Isolation of Cellulolytic Fungi From Sayap-Kinabalu, Sabah. ASEAN Review of Biodiversity and Environmental Conservation (ARBEC).

Khokhar, I; M.S, Haider, and S, Mushtaq.S. 2012. Isolation and Screening of Highly Cellulolytic Filamentous Fungi. J. Appl. Sci. Environ. Manage, Vol. 16 (3) 223-226

Killham, K. 1994. Soil Ecology, 1st eds. Cambridge; New York: Cambridge University Press.

Klein, D. and Eveleigh, E. 1998. Ecology of Trichoderma. In Trichoderma and Gliocladium, pp. 57-69. Edited by C. P. Kubicek and G. E. Harman. London; Bristol, PA: Taylor & Francis.

Prayuwidayati, M. 2009. Pemutusan Ikatan Lignoselulosa Bagas Tebu oleh Isolat Mikrofungi Terseleksi Secara Enzimatis untuk Pembuatan Ransum Ruminansia Berkualitas Tinggi. Unila. PHK-0176 152.118.80.2/opac/themes/green/detail.jsp, diakses 05 Mei 2016.

Rifai, M.A. 1969. A Revision of the Genus Trichoderma. Mycological Papers 116, 1-56.

Samuels, G.J. 1996. Trichoderma: a Review of Biology and Systematics of the Genus. Mycological Research 100:923-935.

Samuels GJ, Dodd SL, Gams W, Castlebury LA, & Petrini O. 2002. Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia 94(1): 146-170.

Shafique, S; Bajwa, R, dan Shafique, S. 2009. Cellulase Biosynthesis By Selected Trichoderma Species. Pak. J. Bot., 41(2): 907-916

Suciatmih, 2001. Test of Lignin and Cellulose Decomposition and Phosphate Solubilization by Soil Fungi of Gunung Halimun. Biodiversitas Taman Nasional Gunung Halimun (I) Jurnal Ilmiah Berita Biologi Edisi Khusus 6(5):685-690.

Tindaon, H . 2008. Pengaruh jamur antagonis Trichoderma harzianum dan pupuk organik untuk mengendalikan patogen tular tanah Sclerotium rolfsii Sacc. pada tanaman kedelai (Glycine max L.) di rumah kasa. Departemen Hama dan Penyakit Tumbuhan. Fakultas Pertanian. Universitas Sumatera Utara.

Agus Purwanto, Universitas Katolik Widya Mandala Surabaya Kampus Kota Madiun

Biologi
Title Isolasi Jamur Selulolitik Trichoderma pada Beberapa Limbah Organik
Issue: Vol. 21 No. 1 (2020): JURNAL AGRITEK
Section Articles
Published: Jul 6, 2020
DOI: https://doi.org/10.33319/agtek.v21i1.70
Author Agus Purwanto
Universitas Katolik Widya Mandala Surabaya Kampus Kota Madiun
Biologi
 PDF
How to Cite
Purwanto, A. (2020). Isolasi Jamur Selulolitik Trichoderma pada Beberapa Limbah Organik. JURNAL AGRI-TEK : Jurnal Penelitian Ilmu-Ilmu Eksakta, 21(1), 42–47. https://doi.org/10.33319/agtek.v21i1.70
 view Abstract : 277      Downloads : 811